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Accueil du site > Equipes > Biologie et Ingénierie du Cartilage (F. Mallein-Gerin) > Thématiques > Voies de la mécanotransduction > Voies de la mécanotransduction

Voies de la mécanotransduction

Project leader : E. Perrier-Groult

People involved in the project : E. Aubert-Foucher, M. Pasdeloup

We have developed a cell system model consisting of chondrocytes embedded within agarose hydrogel and submitted to dynamic compression (figure 1). This model has allowed us to identify new candidate mechanosensitive genes and proteins that are activated (phosphorylated) in response to compression. We are now using this model to explore how mechanical forces are integrated into the BMP-2 pathway. At the tissue level, BMP-2 and mechanical loading are two important parameters for cartilage formation and homeostasis. A better insight into the molecular mechanism by which mechanical forces cross-talk to BMP-2 signals should help to improve cartilage reconstruction in bioreactors, by combining mechanical conditioning and soluble factors such as BMP-2.

Figure 1 : a positive pressure compresses samples (chondrocytes-agarose constructs) in culture plate compression chambers.

Selected publications

1. Bougault C, Aubert-Foucher E, Paumier A, Perrier-Groult E, Huot L, Hot D, Duterque-Coquillaud M, Mallein-Gerin F. (2012) Dynamic compression of chondrocyte-agarose constructs reveals new candidate mechanosensitive genes. PLoS One 7 : e36964.

2. Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F. (2009) Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures.Nat. Protoc. 4 : 928-938.

Collaborations :

A-M Sfarghiu (LaMcoS UMR5259, Insa),

Equipex IVTV

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