Since a decade, bacterial genomic has revealed the widespread
presence of atypical tyrosine-kinases (BYK) and eukaryotic-like
serine/threonine-kinases (STKP). These enzymes have been shown to
participate in the regulation of some important cellular processes.
However, their mode of action is still elusive and underlying regulatory
mechanisms remain largely unknown. In addition, recent high-throughput
and site-specific phosphoproteomic studies lead to the publication of
long lists of bacterial phosphoproteins, suggesting that cellular
functions of BYKs and STKPs could be far more complex than predicted.
Studying BYKs and STKPs represents therefore a promising approach to
decipher the inner workings of the bacterial cell.
research projects focus on the structural and functional
characterization of BYKs and STKPs. The rationale behind our projects is
to decipher phosphorylation networks in which BYKs and STKPs partake in
the bacterial cell, to integrate all components into signaling pathways
and understand how BYKs- and STKPs-mediated protein phosphorylation
governs bacterial virulence and other aspects of cellular regulation.
project should also open new avenues towards specific inhibition of
STKPs and BYKs and thus pave the way towards new antibacterial drugs
affecting growth and virulence of bacterial pathogens.
analytical strategies strongly rely on specific competencies and we use
the synergy created between our team and our collaborators to apply
molecular biology, bacterial genetics, biochemistry, microscopy and
structural biology approaches. Three bacterial models are studied : the
Gram-positive human pathogens Staphylococcus aureus and Streptococcus pneumoniae and the the Gram-negative model bacteria Escherichia coli.